Type II DNA topoisomerases are divided into two families IIA and IIB. In order to investigate the differences in the mechanism of these two prototypical type IA topoisomerases we studied DNA decatenation at the single-molecule level using braids of intact dsDNA and nicked dsDNA with bulges. A The Mechanism Of Type I Dna Topoisomerase The Enzymes Nick A Download Scientific Diagram Describe the general differences between the mechanisms of Type I and Type II topoisomerases. . They use the hydrolysis of ATP unlike Type I topoisomerase. Types IIA and IIB enzymes share homologous B subunits encompassing the ATP-binding site but have non-homologous A subunits catalyzing DNA cleavage. Type-II topoisomerases pass one double-helix segment through another to allow changes in knotting of one mol-ecule or interlinking of double helices. Yan J Magnasco MO Marko JF 2001 Kinetic proofreading can explain the supression of supercoiling of ...
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